DNA damage analysis concerning GSTM1 and GSTT1 gene polymorphism in gold jewellery workers from Peshawar Pakistan. (17th August 2020)

• Record Type: Journal Article
• Authors: Khisroon, Muhammad; Khan, Ajmal; Ayub, Asma; Ullah, Ihsan; Farooqi, Javeed; Ullah, Abid
• Is Part Of: Biomarkers. Volume 25:Number 6(2020)
• Page Start: 483
• Page End: 489
• Publication Date: 2020-08-17
• Subjects: Comet assay -- DNA damage -- gold jewellery workers -- GSTM1 -- GSTT1; Biochemical markers -- Periodicals; 610.28
• DOI: 10.1080/1354750X.2020.1791253
• Languages: English
• ISSNs: 1354-750X

Abstract

Abstract: Purpose: To evaluate the genotoxic effects of gold jewellery fumes and its association with GSTM1 and GSTT1 genetic polymorphisms. Materials and methods: We examined 94 subjects including 54 gold jewellery workers and 40 controls. The DNA damage was evaluated by alkaline comet assay and genotyping by PCR. Results: The mean total comet score (TCS) in gold jewellery workers was significantly higher as compared to the control subjects (128.0 ± 60.6 versus 47.7 ± 21.4; p = 0.0001). Duration of occupational exposure had positive correlation ( r = 0.453, p < 0.01) with DNA damage. Age and tobacco use had significant effects on the TCS of the exposed group as compared to the control group ( p < 0.05). The frequency of the GSTM1-null genotype in the exposed group was significant ( p = 0.004) as compared to the control group. No significant association ( p > 0.05) between the GSTM1 and GSTT1 genotypes and DNA damage was found. Conclusions: Our results suggest that there is increased DNA damage in gold jewellery workers due to their occupational surroundings. Hence there is a strong need to educate the workers about the adverse health effects of potentially hazardous chemicals and highlight the importance of using protective measures.