A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer. (4th May 2017)

• Record Type: Journal Article
• Authors: Zhang, Tao; Liu, Chang; Huang, Shi; Ma, Yuanping; Fang, Jiansong; Chen, Yuanneng
• Other Names: Silvestris Nicola Academic Editor.
• Is Part Of: Gastroenterology research and practice. Volume 2017(2017)
• Publication Date: 2017-05-04
• Subjects: Gastroenterology -- Periodicals; Digestive organs -- Diseases -- Periodicals; 616.33005
• DOI: 10.1155/2017/1526981
• Languages: English
• ISSNs: 1687-6121

Abstract

Abstract : Objective . Here, we aim to investigate the microRNA (miR) profiling in human gastric cancer (GC). Methods . Tumoral and matched peritumoral gastric specimens were collected from 12 GC patients who underwent routine surgery. A high-throughput miR sequencing method was applied to detect the aberrantly expressed miRs in a subset of 6 paired samples. The stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) assay was subsequently performed to confirm the sequencing results in the remaining 6 paired samples. The profiling results were also validated in vitro in three human GC cell lines (BGC-823, MGC-803, and GTL-16) and a normal gastric epithelial cell line (GES-1). Results . The miR sequencing approach detected 5 differentially expressed miRs, hsa-miR-132-3p, hsa-miR-155-5p, hsa-miR-19b-3p, hsa-miR-204-5p, and hsa-miR-30a-3p, which were significantly downmodulated between the tumoral and peritumoral GC tissues. Most of the results were further confirmed by qRT-PCR, while no change was observed for hsa-miR-30a-3p. The in vitro finding also agreed with the results of both miR sequencing and qRT-PCR for hsa-miR-204-5p, hsa-miR-155-5p, and hsa-miR-132-3p. Conclusion . Together, our findings may serve to identify new molecular alterations as well as to enrich the miR profiling in human GC.