Toll-Like Receptor-1 and Receptor-2 and Beta-Defensin in Postcholecystectomy Bile Duct Injury. (8th February 2015)

• Record Type: Journal Article
• Authors: Miranda-Díaz, Alejandra Guillermina; Hermosillo-Sandoval, José Manuel; Villanueva-Pérez, Martha Arisbeth; Román-Pintos, Luis Miguel; García-Iglesias, Trinidad; Rodríguez-Carrizalez, Adolfo Daniel; Cardona-Muñoz, Ernesto Germán
• Other Names: Kahaleh Michel Academic Editor.
• Is Part Of: Gastroenterology research and practice. Volume 2015(2015)
• Publication Date: 2015-02-08
• Subjects: Gastroenterology -- Periodicals; Digestive organs -- Diseases -- Periodicals; 616.33005
• DOI: 10.1155/2015/216129
• Languages: English
• ISSNs: 1687-6121

Abstract

Abstract : Postcholecystectomy bile duct injuries (BDI) produce hepatic cholestasis and cause infection of the biliary tract. The biliary cells participate in secreting cytokines and in expression of immune response receptors. Toll-like receptors (TLRs) conduct signalling and activate the innate and adaptive inflammatory response. The objective was to determine the serum levels of TLR-2 and the expression of TLR-1 and TLR-2 and β -defensin in liver biopsies of postcholecystectomy BDI patients. A transverse, analytical study with 2 groups was done. One group included healthy volunteers (control group) and other included 25 postcholecystectomy BDI patients with complete biliary obstruction. Using the Enzyme-linked Immunosorbent Assay (ELISA) technique, serum levels of TLR-2 were determined, and with immunofluorescence the morphologic analysis of TLR-1 and TLR-2 and β -defensin in liver biopsies of postcholecystectomy BDI patients was performed. The average TLR-2 serum level in the control group was 0.0 pg/mL and in the BDI group, 0.023 ± 0.0045 pg/mL ( P < 0.0001, bilateral Mann Whitney U ). Immunofluorescence was used to determine the expression in liver biopsies, blood vessels, bile ducts, and hepatic parenchyma where 12 hepatic biopsies were positive for TLR-1 with average of 3213057.74 ± 1071019.25 μ m 2 ; and 7 biopsies were positive for β -defensin with an average of 730364.33 ± 210838.02 μ m 2 ; and 6 biopsies positive for TLR-2, obtaining an average of 3354364.24 ± 838591.06 μ m 2 . In conclusion, TLR-1 and TLR-2 and β -defensin play an important role in the innate antimicrobial defense of the hepatobiliary system.