Establishment and Characterization of a Nude Mouse Model of Subcutaneously Implanted Tumors and Abdominal Metastasis in Gastric Cancer. (11th April 2017)

• Record Type: Journal Article
• Authors: Zhu, Yin; Hu, Yi; Cheng, Ming; Zeng, Chun-Yan; Yang, Zhen; Zhou, Xiao-Dong; Chen, Jiang; Lu, Nong-Hua
• Other Names: Fan Daiming Academic Editor.
• Is Part Of: Gastroenterology research and practice. Volume 2017(2017)
• Publication Date: 2017-04-11
• Subjects: Gastroenterology -- Periodicals; Digestive organs -- Diseases -- Periodicals; 616.33005
• DOI: 10.1155/2017/6856107
• Languages: English
• ISSNs: 1687-6121

Abstract

Abstract : A mouse gastric cancer model is an important tool for studying the mechanisms of gastric cancer. To establish subcutaneously implanted tumors, MKN-45 cell suspensions and tumor tissues were implanted into the middle of the right armpit of nude mice. To generate an abdominal metastasis model, MKN-45 cell suspensions and tumor tissue homogenates were implanted into the middle of the lower abdomen. We measured the weights of the nude mice and the longest dimension, shortest dimension, thickness, and volume of the tumor. We also analyzed the rate of tumor formation, the time required for tumor formation, and the number and size of abdominal tumors in the mice. The rates of formation of the subcutaneously implanted tumors were 100%, 0%, and 100% in the nude mice inoculated with 2 × 10 7 cells/mL or 1 × 10 7 cells/mL of the MKN-45 cell suspension or the tumor tissue homogenate (2 × 10 7 cells/mL), respectively. The rates of metastatic abdominal tumor formation were 100%, 50%, and 75% in mice inoculated with 5 × 10 7 cells/mL or 1 × 10 7 cells/mL of the tumor tissue homogenate or the MKN-45 cell suspension (5 × 10 7 cells/mL), respectively. We derived tumor tissues and tumor tissue homogenates from nude mice prior to establishing the subcutaneous model of implanted tumors and the abdominal metastasis model of gastric cancer, respectively.