Abscisic acid signaling negatively regulates nitrate uptake via phosphorylation of NRT1.1 by SnRK2s in Arabidopsis. Issue 3 (3rd March 2021)

• Record Type: Journal Article
• Authors: Su, Hang; Wang, Tian; Ju, Chuanfeng; Deng, Jinping; Zhang, Tianqi; Li, Mengjiao; Tian, Hui; Wang, Cun
• Is Part Of: Journal of integrative plant biology. Volume 63:Issue 3(2021)
• Page Start: 597
• Page End: 610
• Publication Date: 2021-03-03
• Subjects: abscisic acid -- nitrogen deficiency -- NRT1.1 -- SnRK2.2/2.3/2.6; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.13057
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: Nitrogen (N) is a limiting nutrient for plant growth and productivity. The phytohormone abscisic acid (ABA) has been suggested to play a vital role in nitrate uptake in fluctuating N environments. However, the molecular mechanisms underlying the involvement of ABA in N deficiency responses are largely unknown. In this study, we demonstrated that ABA signaling components, particularly the three subclass III SUCROSE NON‐FERMENTING1 (SNF1)‐RELATED PROTEIN KINASE 2S (SnRK2) proteins, function in root foraging and uptake of nitrate under N deficiency in Arabidopsis thaliana . The snrk2.2snrk2.3snrk2.6 triple mutant grew a longer primary root and had a higher rate of nitrate influx and accumulation compared with wild‐type plants under nitrate deficiency. Strikingly, SnRK2.2/2.3/2.6 proteins interacted with and phosphorylated the nitrate transceptor NITRATE TRANSPORTER1.1 (NRT1.1) in vitro and in vivo . The phosphorylation of NRT1.1 by SnRK2s resulted in a significant decrease of nitrate uptake and impairment of root growth. Moreover, we identified NRT1.1 Ser585 as a previously unknown functional site: the phosphomimetic NRT1.1 S585D was impaired in both low‐ and high‐affinity transport activities. Taken together, our findings provide new insight into how plants fine‐tune growth via ABA signaling under N deficiency. Abstract : The core kinases in abscisic acid signaling, SnRK2s, phosphorylate the nitrate transceptor NRT1.1, resulting in a significant decrease of nitrate uptake and impairment of root growth, allowing plants to fine‐tune growth under nitrate deficiency.