Abscisic acid receptors maintain abscisic acid homeostasis by modulating UGT71C5 glycosylation activity. Issue 3 (3rd March 2021)

• Record Type: Journal Article
• Authors: Ma, Yanlin; Cao, Jing; Chen, Qiaoqiao; He, Jiahan; Liu, Zhibin; Wang, Jianmei; Li, Xufeng; Yang, Yi
• Is Part Of: Journal of integrative plant biology. Volume 63:Issue 3(2021)
• Page Start: 543
• Page End: 552
• Publication Date: 2021-03-03
• Subjects: ABA glucosyl ester -- ABA receptors -- glycosylation activity -- ABA homoeostasis -- kinetic analysis; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.13030
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: Uridine diphosphate‐glucosyltransferases (UGTs) maintain abscisic acid (ABA) homeostasis in Arabidopsis thaliana by converting ABA to abscisic acid‐glucose ester (ABA‐GE). UGT71C5 plays an important role in the generation of ABA‐GE. Abscisic acid receptors are crucial upstream components of the ABA signaling pathway, but how UGTs and ABA receptors function together to modulate ABA levels is unknown. Here, we demonstrated that the ABA receptors RCAR12/13 and UGT71C5 maintain ABA homeostasis in Arabidopsis following rehydration under drought stress. Biochemical analyses show that UGT71C5 directly interacted with RCAR8/12/13 in yeast cells, and the interactions between UGT71C5 and RCAR12/13 were enhanced by ABA treatment. Enzyme activity analysis showed that ABA‐GE contents were significantly elevated in the presence of RCAR12 or RCAR13, suggesting that these ABA receptors enhance the activity of UGT71C5. Determination of the content of ABA and ABA‐GE in Arabidopsis following rehydration under drought stress revealed that ABA‐GE contents were significantly higher in Arabidopsis plants overexpressing RCAR12 and RCAR13 than in non‐transformed plants and plants overexpressing RCAR11 following rehydration under drought stress. These observations suggest that RCAR12 and RCAR13 enhance the activity of UGT71C5 to glycosylate excess ABA into ABA‐GE following rehydration under drought stress, representing a rapid mechanism for regulating plant growth and development. Abstract : RCAR12 and RCAR13 enhance the activity of UGT71C5 to glycosylate excess abscisic acid (ABA) into ABA‐GE following rehydration under drought stress, representing a rapid mechanism for regulating ABA homeostasis.