A Simple Route for Purifying Extracellular Poly(3-hydroxybutyrate)-depolymerase from Penicillium pinophilum. (23rd September 2014)

• Record Type: Journal Article
• Authors: Panagiotidou, Elpiniki; Konidaris, Constantinos; Baklavaridis, Apostolos; Zuburtikudis, Ioannis; Achilias, Dimitris; Mitlianga, Paraskevi
• Other Names: Freire Denise Academic Editor.
• Is Part Of: Enzyme research. Volume 2014(2014)
• Publication Date: 2014-09-23
• Subjects: Enzymes -- Periodicals; 572.7
• DOI: 10.1155/2014/159809
• Languages: English
• ISSNs: 2090-0406

Abstract

Abstract : This work proposes the purification of an active and efficient enzyme, extracellular poly(3-hydroxybutyrate) (PHB)-depolymerase, suitable for industrial applications. This is achieved by the application of an easy, fast, and cheap route, skipping the chromatography step. Chromatography with one or two columns is a common step in the purification procedure, which however renders the isolation of the enzyme a time consuming and an expensive process. A strain of the fungus Penicillium pinophilum (ATCC 9644) is used for the isolation of extracellular PHB-depolymerase. The molecular weight of the purified enzyme is about 35 kDa and is estimated by gel electrophoresis (SDS-PAGE, 12% polyacrylamide). The enzymatic activity of the isolated enzyme is determined to be 3.56-fold similar to that found by other researchers that have used chromatography for the isolation. The as-isolated enzyme disintegrates the poly(3-hydroxybutyrate) (PHB) films successfully, as it is demonstrated by the biodegradation test results provided here.