Generation of Arabidopsis thaliana transformants showing the self‐recognition activity of Brassica rapa. (23rd May 2022)

• Record Type: Journal Article
• Authors: Yamamoto, Masaya; Kitashiba, Hiroyasu; Nishio, Takeshi
• Is Part Of: Plant journal. Volume 111:Number 2(2022)
• Page Start: 496
• Page End: 507
• Publication Date: 2022-05-23
• Subjects: self‐incompatibility -- S‐locus receptor kinase -- S‐locus cysteine‐rich protein/S‐locus protein 11 -- Arabidopsis -- Brassica -- Brassicaceae; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.15811
• Languages: English
• ISSNs: 0960-7412

Abstract

SUMMARY: Self‐incompatibility in the Brassicaceae family is governed by SRK and SCR, which are two highly polymorphic genes located at the S ‐locus. Previously, the Arabidopsis lyrata SRK and SCR genes were introduced into Arabidopsis thaliana to generate self‐incompatible lines. However, there are no reports showing that Brassica SRK and SCR genes confer self‐incompatibility in A. thaliana . Doing so would further advance the mechanistic understanding of self‐incompatibility in Brassicaceae. Therefore, we attempted to generate A. thaliana transformants showing the self‐recognition activity of Brassica rapa by introducing BrSCR along with a chimeric BrSRK ( BrSRK chimera, in which the kinase domain of BrSRK was replaced with that of AlSKR‐b ). We found that the BrSRK chimera and BrSCR of B. rapa S‐9 and S‐46 haplotypes, but not those of S‐29, S‐44, and S‐60 haplotypes, conferred self‐recognition activity in A. thaliana . Analyses of A. thaliana transformants expressing mutant variants of the BrSRK‐9 chimera and BrSCR‐9 revealed that mutations at the amino acid residues involved in BrSRK9–BrSCR9 interaction caused defects in the self‐incompatibility response. The method developed in this study for generating self‐incompatible A. thaliana transformants showing B. rapa self‐recognition activity will be useful for analysis of self‐recognition mechanisms in Brassicaceae. Significance Statement: Although self‐incompatible A. thaliana was constructed by introducing SRK and SCR of A. lyrata, it has not been reported that Brassica SRK and SCR genes confer self‐incompatibility in A. thaliana . We generated A. thaliana transformants showing the self‐recognition activity of B. rapa by introducing BrSCR with a BrSRK chimera, in which the kinase domain of BrSRK was replaced with that of AlSKR‐b . Our finding will be useful for analysis of self‐recognition mechanisms in Brassicaceae.