Hsa_circ_0008726 regulates the proliferation, migration, and invasion of trophoblast cells in preeclampsia through modulating the miR‐1290‐LHX6 signaling pathway. Issue 7 (13th June 2022)

• Record Type: Journal Article
• Authors: Zhang, Yongyan; Fang, Shuai; Wang, Jiayi; Chen, Siqian; Xuan, Rongrong
• Is Part Of: Journal of clinical laboratory analysis. Volume 36:Issue 7(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-06-13
• Subjects: hsa_circ_0008726 -- LHX6 -- miR‐1290 -- preeclampsia; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.24540
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract: Background: Preeclampsia (PE) is a serious complication of pregnancy, with a global incidence of about 2%–8%. It is one of the important causes of morbidity and mortality among the pregnant women and perinatal infants. Circular RNA (circRNA) has been confirmed to play an important regulatory role in PE. This study aimed to evaluate the role of hsa_circ_0008726 in the occurrence and development of PE. Methods: The expression of hsa_circ_0008726 in PE placental tissue and blood was detected by qRT‐PCR. CCK‐8, wound closure, and Transwell assay were used to measure cell proliferation, migration, and invasion. Bioinformatics prediction, Western blotting, and dual‐luciferase reporter gene detection were used to explore the mechanism of hsa_circ_0008726 in HTR8/SVneo cells. Results: The expression level of circ_0008726 in the placental tissue and blood samples of PE patients was significantly higher than that of normal controls. The overexpression of circ_0008726 can significantly inhibit the proliferation, migration, and invasion ability of HTR‐8/SVneo cells. While the silence of circ_0008726 showed an opposite effect. Furthermore, hsa_circ_0008726 can modulate the expression of LHX6 by adsorbing miR‐1290. Conclusion: Hsa_circ_000872 can regulate LHX6 by adsorbing miR‐1290 to inhibit PE progression, thus establishing hsa_circ_000872 as a potential target for predicting and treating PE. Abstract : In this study, we screened out several differentially expressed circRNAs by mining and sorting out PE gene chip data. Thereafter, by using different assays, we have found that hsa_circ_0008726 is highly expressed in PE. At the same time, our findings indicate that hsa_circ_0008726 can effectively regulate LHX6 through modulating miR‐1290 to potentially inhibit the occurrence and development of PE in vitro. Overall, findings of our research provide new insights into the pathogenesis of PE.