Super‐Resolution Vibrational Imaging Using Expansion Stimulated Raman Scattering Microscopy. Issue 20 (6th May 2022)

• Record Type: Journal Article
• Authors: Shi, Lixue; Klimas, Aleksandra; Gallagher, Brendan; Cheng, Zhangyu; Fu, Feifei; Wijesekara, Piyumi; Miao, Yupeng; Ren, Xi; Zhao, Yongxin; Min, Wei
• Is Part Of: Advanced science. Volume 9:Issue 20(2022)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2022-05-06
• Subjects: chemical imaging -- expansion microscopy -- highly multiplexed nanoscale imaging -- metabolic imaging -- Raman nanoscopy; Science -- Periodicals; 505
• DOI: 10.1002/advs.202200315
• Languages: English
• ISSNs: 2198-3844

Abstract

Abstract: Stimulated Raman scattering (SRS) microscopy is an emerging technology that provides high chemical specificity for endogenous biomolecules and can circumvent common constraints of fluorescence microscopy including limited capabilities to probe small biomolecules and difficulty resolving many colors simultaneously. However, the resolution of SRS microscopy remains governed by the diffraction limit. To overcome this, a new technique called molecule anchorable gel‐enabled nanoscale Imaging of Fluorescence and stimulated Raman scattering microscopy (MAGNIFIERS) that integrates SRS microscopy with expansion microscopy (ExM) is described. MAGNIFIERS offers chemical‐specific nanoscale imaging with sub‐50 nm resolution and has scalable multiplexity when combined with multiplex Raman probes and fluorescent labels. MAGNIFIERS is used to visualize nanoscale features in a label‐free manner with CH vibration of proteins, lipids, and DNA in a broad range of biological specimens, from mouse brain, liver, and kidney to human lung organoid. In addition, MAGNIFIERS is applied to track nanoscale features of protein synthesis in protein aggregates using metabolic labeling of small metabolites. Finally, MAGNIFIERS is used to demonstrate 8‐color nanoscale imaging in an expanded mouse brain section. Overall, MAGNIFIERS is a valuable platform for super‐resolution label‐free chemical imaging, high‐resolution metabolic imaging, and highly multiplexed nanoscale imaging, thus bringing SRS to nanoscopy. Abstract : This work develops a new technique called MAGNIFIERS that integrates stimulated Raman scattering (SRS) microscopy with expansion microscopy for super‐resolution vibrational imaging. MAGNIFIERS revitalizes a full spectrum of utilities of SRS imaging into the sub‐diffraction regime with an effective resolution below 50 nm and general sample compatibility. Specifically, it supports super‐resolution label‐free chemical imaging, high‐resolution metabolic imaging, and highly multiplexed nanoscale imaging.