AtNSF regulates leaf serration by modulating intracellular trafficking of PIN1 in Arabidopsis thaliana. Issue 4 (5th April 2021)

• Record Type: Journal Article
• Authors: Tang, Li Ping; Yang, Yi; Wang, Hui; Li, Lixin; Liu, Le; Liu, Yu; Yuan, Jinfeng; Zhao, Xiang Yu; Palme, Klaus; Su, Ying Hua; Li, Xugang
• Is Part Of: Journal of integrative plant biology. Volume 63:Issue 4(2021)
• Page Start: 737
• Page End: 755
• Publication Date: 2021-04-05
• Subjects: AtNSF -- Auxin -- CUC2 -- leaf serration -- PIN1 -- vesicle trafficking; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.13043
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: In eukaryotes, N ‐ethylmaleimide‐sensitive factor (NSF) is a conserved AAA+ATPase and a key component of the membrane trafficking machinery that promotes the fusion of secretory vesicles with target membranes. Here, we demonstrate that the Arabidopsis thaliana genome contains a single copy of NSF, AtNSF, which plays an essential role in the regulation of leaf serration. The AtNSF knock‐down mutant, atnsf‐1, exhibited more serrations in the leaf margin. Moreover, polar localization of the PIN‐FORMED1 (PIN1) auxin efflux transporter was diffuse around the margins of atnsf‐1 leaves and root growth was inhibited in the atnsf‐1 mutant. More PIN1‐GFP accumulated in the intracellular compartments of atnsf‐1 plants, suggesting that AtNSF is required for intracellular trafficking of PIN between the endosome and plasma membrane. Furthermore, the serration phenotype was suppressed in the atnsf‐1 pin1‐8 double mutant, suggesting that AtNSF is required for PIN1‐mediated polar auxin transport to regulate leaf serration. The CUP‐SHAPED COTYLEDON2 ( CUC2 ) transcription factor gene is up‐regulated in atnsf‐1 plants and the cuc2‐3 single mutant exhibits smooth leaf margins, demonstrating that AtNSF also functions in the CUC2 pathway. Our results reveal that AtNSF regulates the PIN1‐generated auxin maxima with a CUC2‐mediated feedback loop to control leaf serration. Abstract : N‐ethylmaleimide‐sensitive factor (NSF) promotes the fusion of secretory vesicles in eukaryotes. In Arabidopsis, AtNSF negatively regulates leaf serration by affecting trafficking of the PIN‐FORMED1 auxin transporter and interfering with the PIN‐FORMED1 and CUP‐SHAPED COTYLEDON2‐mediated feedback loop for leaf serration.