Water-mediated structural rearrangement establishes active conformation of caspases for apoptosis and inflammation. Issue 13 (21st July 2022)

• Record Type: Journal Article
• Authors: Sharma, Anjaney; Gogoi, Prerana; Chandravanshi, Monika; Kanaujia, Shankar Prasad
• Is Part Of: Journal of biomolecular structure & dynamics. Volume 40:Issue 13(2022)
• Page Start: 6013
• Page End: 6026
• Publication Date: 2022-07-21
• Subjects: Apoptotic caspase -- inflammatory caspase -- invariant water molecules -- molecular dynamics -- protease -- zymogen; Biomolecules -- Periodicals; Molecular structure -- Periodicals; Molecular Biology -- Periodicals; Biomechanics -- Periodicals; 572
• DOI: 10.1080/07391102.2021.1875884
• Languages: English
• ISSNs: 0739-1102

Abstract

Abstract: Caspases are c ysteine-dependent asp artate-specific proteases that play a crucial role in apoptosis (or programmed cell death) and inflammation. Based on their function, caspases are majorly categorized into apoptotic (initiator/apical and effector/executioner) and inflammatory caspases. Caspases undergo transition from an inactive zymogen to an active caspase to accomplish their function. This transition demands structural rearrangements which are most prominent at the active site loops and are imperative for the catalytic activity of caspases. In effector caspase-3, the structural rearrangement in the active site loop is shown to be facilitated by a set of invariant water (IW) molecules. However, the atomic details involving their role in stabilizing the active conformation have not been reported yet. Moreover, it is not known whether water molecules are essential for the active conformation in all caspases. Thus, in this study, we located IW molecules in initiator, effector, and inflammatory caspases to understand their precise role in rendering the structural arrangement of active caspases. Furthermore, IW molecules involved in anchoring the fragments of the protomer and rendering regulated flaccidity to caspases were identified. Location and identification of IW molecules interacting with amino acid residues involved in establishing the active conformation in the caspases might facilitate the design of potent inhibitors during up-regulated caspase activity in neurodegenerative and immune disorders. Communicated by Ramaswamy H. Sarma