Reuse of wheat flour liquid waste for enzymatic hydrolysis to yield glucose-derived bioethanol. Issue 1 (31st December 2022)

• Record Type: Journal Article
• Authors: Sari, N.K.; Purbasari, I.Y.; Anggoro, P.W.; Jamari, J.; Bayuseno, A.P.
• Editors: Arellano-Garcia, Harvey
• Is Part Of: Cogent engineering. Volume 9:Issue 1(2022)
• Publication Date: 2022-12-31
• Subjects: wheat flour liquid waste -- pH -- amylase and maltose enzymes -- surface response methodology (SRM); Engineering -- Periodicals; Technology -- Periodicals; Engineering; Technology; Periodicals; 620
• DOI: 10.1080/23311916.2022.2101229
• Languages: English
• ISSNs: 2331-1916

Abstract

Abstract: Wheat flour liquid waste (WF-LW) is a potential biomass that is chemically pre-treated before being enzymatically hydrolyzed to produce glucose-derived bioethanol. This study investigated the effects of pH on the pretreatment process and the subsequent hydrolysis of WF-LW in the presence of amylase (AE) and maltose enzymes (ME). In the experiment, WF-LW at varying volumes from 200 to 1000 mL was treated with 5 to 25 mL of HCl and stirred for 30 minutes and at the temperature of 40°C. Impurities were removed prior to hydrolysis. Following that, a hydrolysis with an AE of 11% w/v and ME of 9% w/v resulted in a glucose content of 24.9% v/v. Moreover, 0.859 mL of HCl was added to achieve the best results, which were then optimized using SRM (surface response methodology) at a pH of 8.85 in a WF-LW volume of 1165.69 mL. The optimization of the pH and fermentation process in the presence of amylase and maltase enzymes resulted in increased glucose production and a decrease in the LW-TW treatment volume, which was 651.426 mL. These discoveries would become an appropriate approach for the subsequent fermentation process, resulting in the highest glucose levels possible.