Visualization of the photodegradation of a therapeutic drug by chemometric-assisted fluorescence spectroscopy. Issue 32 (19th July 2022)

• Record Type: Journal Article
• Authors: Tanioka, Masaru; Ebihana, Tsugumi; Uraguchi, Manae; Shoji, Haruka; Nakamura, Yuka; Ueda, Rina; Ogura, Shota; Wakiya, Yoshifumi; Obata, Tohru; Ida, Takahiro; Horigome, Jun; Kamino, Shinichiro
• Is Part Of: RSC advances. Volume 12:Issue 32(2022)
• Page Start: 20714
• Page End: 20720
• Publication Date: 2022-07-19
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra03534k
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Drug quality and assurance changes with time under the influence of a variety of environmental factors, such as light, temperature, and moisture. Abstract : The fluorescence spectral fingerprint, also known as the excitation-emission matrix (EEM), is used to assess and visualize therapeutic drug photodegradation in combination with chemometrics. Examination of EEM-parallel factor analysis (PARAFAC) data showed that an individual component was easily separated from a mixture of photogenerated products of a heterocyclic pharmacophore, in this case, phenothiazine drugs (PTZs). Detailed investigations of both structure–EEM relationships and kinetics revealed that the components extracted from EEM–PARAFAC could be quantitatively attributed to such photogenerated products as phenothiazine sulfoxide and carbazole derivatives. EEM in combination with principal component analysis (PCA) could be used as a mapping tool to visualize information of the photodegradation process of PTZs. We also assessed the photostability of various types of PTZs containing side chains by using validated EEM–PARAFAC methodology.