A novel metal–organic framework of Ba–hemin with enhanced cascade activity for sensitive glucose detection. Issue 32 (15th July 2022)

• Record Type: Journal Article
• Authors: Yi, Jintao; Han, Xianqin; Gao, Fengying; Cai, Le; Chen, Ying; Deng, Xiulong; Li, Xun; Xue, Jun; Zhou, Hui
• Is Part Of: RSC advances. Volume 12:Issue 32(2022)
• Page Start: 20544
• Page End: 20549
• Publication Date: 2022-07-15
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra02778j
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : The Ba–hemin@GOX composite is used for sensitive glucose detection. Abstract : Early glucose detection is important in both healthy people and diabetic patients. Glucose biosensing based on glucose oxidase (GOX) is a common method. However, native proteins are mostly membrane impermeable and are prone to degradation in complex sample environments. Herein, we report a facile one-step biomineralization method by simply mixing aqueous solutions of hemin and barium nitrate with glucose oxidase (GOX) to form Ba–hemin@GOX composites. Glucose (Glu) is introduced through self-driven sampling to trigger the GOX-catalysed production of hydrogen peroxide, which could help the subsequent 3, 3′, 5, 5′-tetramethylbenzidine (TMB) oxidation reaction catalysed by Ba–hemin to yield the blue-coloured product. The sensor exhibited a detection limit as low as 3.08 μM. The operability and accuracy of the Ba–hemin@GOX biosensor were confirmed by the quantitative determination of glucose in real samples, such as tap water, serum and drinks. Moreover, the Ba–hemin@GOX-based colorimetric biosensor showed good selectivity, storage stability and recoverability. The experimental results reveal that a GOX activity of more than 90% was still maintained even after being incubated at 60 °C for 30 minutes, and Ba–hemin@GOX could be reused for glucose detection at least six times. Even after 30 days of storage, the relative activity was still more than 90%. Overall, the developed Ba–hemin@GOX biosensor provides a valuable and general platform for applications in colorimetric biosensing and medical diagnostics.