Immunological quantitative detection of dicofol in medicinal materials. Issue 15 (29th June 2022)

• Record Type: Journal Article
• Authors: Lin, Lu; Xu, Xinxin; Song, Shanshan; Xu, Liguang; Zhu, Yingyue; Kuang, Hua; Liu, Liqiang; Xu, Chuanlai
• Is Part Of: Analyst. Volume 147:Issue 15(2022)
• Page Start: 3478
• Page End: 3485
• Publication Date: 2022-06-29
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/d2an00462c
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : A quantitative immunochromatographic strip assay was developed for the rapid detection of dicofol in medicinal materials. Abstract : In this study, a highly specific and sensitive monoclonal antibody against dicofol (DIC) was prepared and used to create a colloidal gold immunochromatographic strip (ICS) for the quantitative analysis of DIC in liquorice and Angelica sinensis samples. We found that for both the liquorice and Angelica sinensis samples, the cut-off value for the ICS was 500 ng g −1, respectively, and using a colloidal gold test strip reader, the half-maximal inhibitory concentrations (IC50 ) were found to be 35 ng g −1 and 88 ng g −1, the limits of detection were 2 ng g −1 and 3 ng g −1, and the linear detection scopes (IC20 –IC80 ) were from 6 to 201 ng g −1 and from 10 to 819 ng g −1, respectively. In the recovery test, we found that recoveries were within the range of 90.6% to 97.2% and the coefficients of variation were less than 8.6% in the intra-assay and inter-assay for the liquorice samples and the recoveries ranged from 90.8% to 97.3% and the coefficients of variation were less than 9.1% in the intra-assay and inter-assay for the Angelica sinensis samples. The established ICS assay could be used for the rapid quantitative screening of chemicals in Chinese traditional medicinal materials.