Functional pleiotropism, diversity and redundancy of Salvia miltiorrhiza Bunge JAZ family proteins in jasmonate-induced tanshinone and phenolic acid biosynthesis. Issue 1 (25th July 2022)

• Record Type: Journal Article
• Authors: Ma, Pengda; Pei, Tianlin; Lv, Bingbing; Wang, Mei; Dong, Juane; Liang, Zongsuo
• Is Part Of: Horticulture research. Volume 9:Issue 1(2022)
• Publication Date: 2022-07-25
• Subjects: Salvia miltiorrhiza -- tanshinone -- salvianolic acid B -- jasmonate -- JAZs; Horticulture -- Research -- Periodicals; 635.072
• DOI: 10.1093/hr/uhac166
• Languages: English
• ISSNs: 2052-7276

Abstract

Abstract: Jasmonate (JA) signaling regulates plant growth and development, biotic and abiotic stress tolerance, and primary and secondary metabolism biosynthesis. It is extensively modulated by JA-ZIM-domain ( JAZ ) family genes. In previous work, we obtained 9 SmJAZ genes of Salvia miltiorrhiza and proved that SmJAZ8 was the core repressor of JA-induced tanshinone and phenolic acid biosynthesis. Here, we demonstrate that SmJAZ3 and SmJAZ4 act as repressors of JA-induced biosynthesis of tanshinones and salvianolic acid B (Sal B). This suggests that SmJAZ3/4 redundancy of functional of the tanshinone and Sal B biosynthesis. SmJAZ1/2/5/6/9 are activators for JA-induced tanshinones biosynthesis and repressors for the JA-induced Sal B biosynthesis. This demonstrates the redundancy and diversity of SmJAZ1/2/5/6/9 functions. Besides, SmJAZ10 only inhibited JA-induced Sal B synthesis, but had no effect on the synthesis of tanshinone. Two-hybrid screening (Y2H) showed that SmJAZs formed homologous or heterogeneous dimers. Y2H and firefly luciferase complementation imaging (LCI) assays revealed that SmJAZs also formed a complex regulatory network with SmMYC2a, SmMYC2b, SmMYB39, and SmPAP1. Quantitative reverse transcription-PCR (qRT-PCR) indicated that SmJAZs regulated each other at the transcriptional level. Herein, we prove that SmJAZ s have functional pleiotropism, diversity and redundancy in JA-induced tanshinones and phenolic acids biosynthesis. This study provides an important clue for further understanding the inherent biological significance and molecular mechanisms of the JAZ family as the gene number increases during plant evolution.