In vitroactivity of bedaquiline against rapidly growing nontuberculous mycobacteria. Issue 8 (August 2017)

• Record Type: Journal Article
• Authors: Aguilar‐Ayala, Diana A.; Cnockaert, Margo; André, Emmanuel; Andries, Koen; Gonzalez‐Y‐Merchand, Jorge A.; Vandamme, Peter; Palomino, Juan Carlos; Martin, Anandi
• Is Part Of: Journal of medical microbiology. Volume 66:Issue 8(2017)
• Publication Date: 2017-08
• Subjects: resistance to bedaquiline -- nontuberculous mycobacteria -- atpE mutation -- Mycobacterium flavescens -- minimum inhibitory concentration -- minimum bactericidal concentration; Medical microbiology -- Periodicals; 616.9041
• DOI: 10.1099/jmm.0.000537
• Languages: English
• ISSNs: 0022-2615

Abstract

Abstract : Abstract : Bedaquiline (BDQ) has been proven to be effective in the treatment of multidrug‐resistant tuberculosis. We hypothesized that BDQ could be a potential agent to treat nontuberculous mycobacterial (NTM) infection. The objective of this study was to evaluate the in vitro activity of BDQ against rapidly growing mycobacteria by assessing the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) against 18 NTM strains. For MIC determination we performed the resazurin microtitre assay broth dilution, and for the MBC the c.f.u. was determined. BDQ exhibited a strong inhibitory effect against most NTM tested; however, for some NTM strains the MBC was significantly higher than the MIC. A new finding is that Mycobacterium flavescens has a mutation in the gene atpE associated with natural resistance to BDQ. These preliminary promising results demonstrate that BDQ could be potentially useful for the treatment of NTM.