Construction of pH/reduction dual responsive MSN-HAgel containing HApt for tumor targeting carriers. Issue 30 (29th June 2022)

• Record Type: Journal Article
• Authors: Liu, Yehong; Chen, Miaoxin; Li, Gaoyang; Xu, Shouhong; Liu, Honglai
• Is Part Of: RSC advances. Volume 12:Issue 30(2022)
• Page Start: 19063
• Page End: 19071
• Publication Date: 2022-06-29
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra02290g
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : A dual-responsive MSN-based nanocarrier for the combination of gene therapy and chemotherapy in cancer treatments. Abstract : In this study, a pH/reduction dual responsive carrier containing 42nt-nucleic acid HApt based on mesoporous silica nanoparticles (MSNs) was designed. Two kinds of low molecular weight oligomeric hyaluronic acid (HA) were used to graft onto MSN for better drug encapsulation. Crosslinked MSN-HA3000 gel and MSN-HA11000 gel were prepared by crosslinking the HA chain through the sulfhydrylization of the carboxyl group on the HA side chain. An appropriate amount of sulfhydryl nucleic acid (HApt-SH) was added during the crosslinking reaction, which realized the targeting ability and apoptosis function to cancer cells overexpressing the HER2 receptor. Crosslinked HA had a good effect on decreasing the side effect of DOX that the drug leakage was less than 20% under a normal body environment. However, it could realize rapid and efficient drug release in a tumor environment. As to the release of HApt, it exhibited a good response to GSH. The cytotoxicity test showed that HApt contained in HAgel had a great targeting effect and significant cytotoxicity to SKBR3 cells. As a whole, this MSN-HAgel enabled the combination of gene therapy and chemotherapy, showing the synergistic effect of "1 + 1 > 2", providing a novel idea for cancer treatments.