Furostanol saponins from Asparagus racemosus as potential hypoglycemic agents. (September 2022)

• Record Type: Journal Article
• Authors: Pandey, Alka Raj; Ahmad, Shadab; Singh, Suriya Pratap; Mishra, Anjali; Bisen, Amol Chhatrapati; Sharma, Gaurav; Ahmad, Ishbal; Shukla, Sanjeev K.; Bhatta, Rabi Sankar; Kanojiya, Sanjeev; Tamrakar, Akhilesh Kumar; Sashidhara, Koneni V.
• Is Part Of: Phytochemistry. Volume 201(2022)
• Publication Date: 2022-09
• Subjects: Asparagus racemosus -- Asparagaceae -- Type 2 diabetes mellitus (T2DM) -- GLUT4 translocation -- AMPK signaling Pathway -- Antihyperglycaemic -- Furostanol saponin; Botanical chemistry -- Periodicals; Biochemistry -- Periodicals; Botany -- Periodicals; Chimie végétale -- Périodiques; 572.2
• DOI: 10.1016/j.phytochem.2022.113286
• Languages: English
• ISSNs: 0031-9422

Abstract

Abstract: Bioactivity guided phytochemical investigation led to isolation of six undescribed furostanol saponins, furoasparoside A-F along with five known compounds, gallic acid, methyl gallate, quercetin-3-O- β -glucopyranoside, liquiritigenin 4 ׳ -O- β- apiofuranosyl-(1 → 2)- β -glucopyranoside and β -glucogallin for the first time from the roots of Asparagus racemosus . Isolated saponins were screened for their antidiabetic potential in L6-GLUT4 myc myotubes in vitro followed by an in vivo evaluation in streptozocin-induced diabetic rats and db/db mice. Furoasparoside E produced a notable decrease in the postprandial blood glucose profile, in leptin receptor-deficient db/db mice, type 2 diabetes model. The effect of furoasparoside E on GLUT4 translocation was found to be mediated by the AMPK-dependent signaling pathway in L6-GLUT4 myc myotubes. Moreover, it emerged as a stable plant metabolite with higher bioavailability and efficacy in in vivo pharmacokinetic studies. Therefore, these studies indicated that furoasparoside E may serve as a propitious lead for the management of type 2 diabetes and its secondary complications from natural source. Graphical abstract: Image 1 Highlights: Six undescribed furostanol saponins were isolated from Asparagus racemosus. Furoasparoside E showed significant in vitro increase in surface level of GLUT4 myc at 10 μM. It showed comparable blood glucose lowering effect with metformin in STZ induced diabetic rats. It demonstrated improvement in glucose metabolism in db/db mice model. Furoasparoside E followed activation of AMPK-dependent signaling pathway.