Determination of aflatoxin B1 in Pixian Douban based on aptamer magnetic solid-phase extraction. Issue 30 (6th July 2022)

• Record Type: Journal Article
• Authors: Zeng, Chaoyi; Xu, Chi; Tian, Hongyun; Shao, Kun; Song, Yaning; Yang, Xiao; Che, Zhenming; Huang, Yukun
• Is Part Of: RSC advances. Volume 12:Issue 30(2022)
• Page Start: 19528
• Page End: 19536
• Publication Date: 2022-07-06
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d2ra02763a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : An aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB1 from a Pixian Douban sample. It was developed based on aptamer–Fe3 O4 @SiO2 –NH2 with subsequent ELISA validation, showing an efficient result. Abstract : Aflatoxin B1 (AFB1 ) is considered as the most prevalent and toxic mycotoxin in food, and is the indispensable index in the monitoring of Pixian Douban, a traditional chinese fermented bean paste from Sichuan. However, the effeciency of AFB1 detection in Pixian Douban is influenced by the traditional extraction, which is usually complex and time consuming. Therefore, an aptamer-based magnetic solid-phase extraction method was designed for the pretreatment of AFB1 in this sample, for which Fe3 O4 was synthesized via the solvothermal method and then a Fe3 O4 @SiO2 –NH2 with a core–shell structure was prepared, followed by an AFB1 -aptamer attachment. The validation was performed via an enzyme-linked immunosorbent assay and compared with HPLC-MS/MS. The linearity range of this method was 0.5–2.0 ng mL −1 with R 2 of 0.981, and recoveries of AFB1 ranged from 80.19% to 113.92% with RSDs below 7.28% with no significant differences compared to HPLC-MS/MS. The three-time reusability efficiencies of aptamer-MNPs were averaged at 78.24%. The results proved that aptamer-MNPs were high-performance adsorbents for extracting and enriching AFB1, facilitating quick and effective detection of AFB1 in Pixian DouBan samples.