Effect of biomimetic mineralization on enamel and dentin: A Raman and EDX analysis. Issue 9 (September 2019)

• Record Type: Journal Article
• Authors: Guentsch, Arndt; Fahmy, Mina D.; Wehrle, Constanze; Nietzsche, Sandor; Popp, Jürgen; Watts, David C.; Kranz, Stefan; Krafft, Christoph; Sigusch, Bernd W.
• Is Part Of: Dental materials. Volume 35:Issue 9(2019)
• Page Start: 1300
• Page End: 1307
• Publication Date: 2019-09
• Subjects: Biomimetic -- Mineralization -- Enamel -- Dentin -- Raman -- EDX; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2019.05.025
• Languages: English
• ISSNs: 0109-5641

Abstract

Highlights: A biomimetic-mineralization kit utilized gelatin gels loaded with calcium and phosphate. The mineralization of enamel-like layers was observed on enamel and dentin samples. Fluoride ions penetrated in enamel and dentin. The degree of mineralization was increased in both, treated enamel and dentin samples. Abstract: Objective: To investigate the effect of an experimental biomimetic mineralization kit (BIMIN) on the chemical composition and crystallinity of caries-free enamel and dentin samples in vitro . Methods: Enamel and dentin samples from 20 human teeth (10 for enamel; 10 for dentin) were divided into a control group without treatment and test samples with BIMIN treatment. Quantitative analysis of tissue penetration of fluoride, phosphate, and calcium was performed using energy-dispersive X-ray spectroscopy (EDX). Mineralization depth was measured by Raman spectroscopy probing the symmetric valence vibration near 960 cm −1 as a marker for crystallinity. EDX data was statistically analyzed using a paired t -test and Raman data was analyzed using the Student's t -test. Results: EDX analysis demonstrated a penetration depth of fluoride of 4.10 ± 3.32 μm in enamel and 4.31 ± 2.67 μm in dentin. Calcium infiltrated into enamel 2.65 ± 0.64 μm and into dentin 5.58 ± 1.63 μm, while the penetration depths for phosphate were 4.83 ± 2.81 μm for enamel and 6.75 ± 3.25 μm for dentin. Further, up to 25 μm of a newly mineralized enamel-like layer was observed on the surface of the samples. Raman concentration curves demonstrated an increased degree of mineralization up to 5–10 μm into the dentin and enamel samples. Significance: Biomimetic mineralization of enamel and dentin samples resulted in an increase of mineralization and a penetration of fluoride into enamel and dentin.