Hyaluronic microparticle-based biomimetic artificial neighbors of cells for three-dimensional cell culture. (15th October 2022)

• Record Type: Journal Article
• Authors: Jeong, Ji-Eun; Han, Sang-Soo; Shim, Hye-Eun; Kim, Woojin; Lee, Byoung-Seok; Kim, Yong-Jin; Kang, Sun-Woong
• Is Part Of: Carbohydrate polymers. Volume 294(2022)
• Publication Date: 2022-10-15
• Subjects: Spheroid -- Hyaluronic acid particle -- 3D cell culture -- Scaffold-based system -- Scaffold-free system; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2022.119770
• Languages: English
• ISSNs: 0144-8617

Abstract

Abstract: 3D spheroids, which have the potential to bridge the gap between 2D cell culture and native tissue, are used as tissue models in many applications, particularly in cancer, stem cell, and pharmaceutical research. A considerable amount of effort has focused on the development of more relevant physiological models. However, spheroids still have limitations in that they cannot replicate the components and structure of the ECM in the natural environment. In this study, we proposed new concept of scaffold-based techniques for the generation of spheroids. Spheroids were successfully generated by single cell or small number of aggregated cells between HA particles. The size of each spheroid was uniform, a necrotic core didn't form, and the system showed high viability. The expression levels of the proteins and genes required to maintain cell-specific functions increased. Thus, our system provides more physiologically relevant models and could be applied to regenerative medicine or drug screening. Graphical abstract: Unlabelled Image Highlights: Biomimetic artificial neighbors (BAN) system ideally control the size of spheroids with a higher cell density. Biomimetic artificial neighbors (BAN) system can provide the sufficient oxygen and nutrient into the spheroids. Biomimetic artificial neighbors (BAN) can generate the homogeneous spheroids with enhanced cellular functions.