The influence of experimental bioactive glasses on pulp cells behavior in vitro. Issue 10 (October 2020)

• Record Type: Journal Article
• Authors: Mocquot, Caroline; Colon, Pierre; Fernando, Delihta; Jackson, Phil; Pradelle-Plasse, Nelly; Grosgogeat, Brigitte; Attik, Nina
• Is Part Of: Dental materials. Volume 36:Issue 10(2020)
• Page Start: 1322
• Page End: 1331
• Publication Date: 2020-10
• Subjects: Biocompatibility -- Bioactive glasses -- Mesoporous -- Dental pulp cells -- Mineralization; Dentistry -- Periodicals; Dental materials -- Periodicals; 617.695
• DOI: 10.1016/j.dental.2020.07.006
• Languages: English
• ISSNs: 0109-5641

Abstract

Abstract: Objectives: To assess in vitro the effect of experimental mesoporous BAG, on human dental pulp cells (hDPCs) behavior in terms of cytocompatiblity and bioactivity via mineralization potential. Methods: Fine (FP) and large particles (LP) of a fixed BAG composition named 0NaMBG have been elaborated by a sol-gel process. In vitro assessment was achieved on cultured primary hDPCs using indirect contact. The effect of the concentration of 800 μg/mL on cell metabolic activity and cytotoxicity were examined by performing Alamar blue and crystal violet assays. Alizarin Red staining was used to detect and quantify the formation of mineralized nodules and ALP activity was colourimetrically quantified. The expression of Odontogenic markers: DMP-1 and osteopontin (OPN) expression and cell morphology was evaluated by confocal microscopy. Results: According to the Alamar blue and crystal violet assay, 0NaMBG samples were non-cytotoxic. Cells treated with 0NaMBG particles expressed higher metabolic activity than control cells, especially for LP. Both FP and LP significantly increased both extra and intra cellular ALP activity. hDPCs exhibited good cell spreading and adhesion in the presence of FP and LP extracts by confocal imaging. Further, Alizarin red S assay demonstrated more mineralization nodules and significant enhancement of the extracellular calcium deposition when cells were interfaced with both FP and LP compared to the control cells. Moreover, LP extracts enhanced the production and secretion of odontogenic markers: dentin matrix protein 1 (DMP-1) and osteopontin. Significance: LP have a higher surface area and pore volume, which could explain their greater bioactivity in contact with pulp cells. The clinical relevance of these findings implicate that 0NaMBG could be used as fillers in dental therapeutic materials suitable for dentin and/or pulp tissues preservation.