Cdk1 negatively regulates the spindle localization of Prc1 in mouse oocytes. (21st September 2020)

• Record Type: Journal Article
• Authors: Nishiyama, Sui; Yoshida, Shuhei; Kitajima, Tomoya S.
• Is Part Of: Genes to cells. Volume 25:Number 10(2020)
• Page Start: 685
• Page End: 694
• Publication Date: 2020-09-21
• Subjects: meiosis -- oocyte -- spindle; Cytogenetics -- Periodicals; Cells -- Mechanical properties -- Periodicals; Molecular genetics -- Periodicals; Genes -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Biomechanics -- Periodicals; 571.6
• DOI: 10.1111/gtc.12803
• Languages: English
• ISSNs: 1356-9597

Abstract

Abstract: Chromosome segregation requires the formation of a bipolar spindle. The timely bipolarization of the acentrosomal spindle during meiosis I in mouse oocytes depends on the antiparallel microtubule crosslinker Prc1. How Prc1 is regulated in oocytes remains poorly understood. In this study, we show that the kinase Cdk1 negatively regulates the spindle localization of Prc1 in mouse oocytes. The acute inhibition of Cdk1 activity led to excessive localization of Prc1 at the spindle and kinetochores, whereas the overactivation of Cdk1 had opposite effects. The overexpression of Prc1 carrying mutations at Cdk1‐mediated phosphorylation sites increased its localization to the spindle, accelerated spindle bipolarization and caused spindle‐checkpoint‐dependent arrest at metaphase I. Overactivation of Cdk1 delayed spindle bipolarization, which was reversed by the overexpression of a phospho‐mutant form but not the wild‐type form of Prc1. These results suggest that Cdk1‐mediated phosphorylation negatively regulates Prc1 localization to ensure the timely bipolarization of the acentrosomal spindle during meiosis I in mammalian oocytes. Abstract : Oocytes require timely assembly of the acentrosomal spindle to produce eggs with the correct number of chromosomes. This article reports that Cdk1‐mediated phosphorylation negatively regulates Prc1, an anti‐parallel microtubule cross‐linker that promotes spindle assembly, in mouse oocytes.