Circular RNA circDLGAP4 is involved in lung cancer development through modulating microRNA-143/CDK1 axis. Issue 16 (17th August 2020)

• Record Type: Journal Article
• Authors: Wang, Bin; Hua, Peiyan; Zhao, Bin; Li, Jindong; Zhang, Yan
• Is Part Of: Cell cycle. Volume 19:Issue 16(2020)
• Page Start: 2007
• Page End: 2017
• Publication Date: 2020-08-17
• Subjects: Lung cancer -- circDLGAP4 -- cyclin-dependent kinase 1 -- microRNA-143; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2020.1786649
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: To investigate the role of circular RNA DLGAP4 (circDLGAP4) in lung cancer. circDLGAP4 expression was detected in lung cancer tissues and cell lines by PCR. The correlation between circDLGAP4 and clinicopathological characteristics of lung cancer patients was investigated. Moreover, the influences of depression of circDLGAP4 on the biological processes biological processes of lung cancer cells were explored in vitro . In addition, whether circDLGAP4 regulated lung cancer cell biological processes by sponging microRNA-143 (miR-143) to regulate cyclin-dependent kinase 1 (CDK1) expression was explored and verified in another lung cell line. CircDLGAP4 expression was remarkably elevated in lung cancer tissues and was significantly corrected with TNM stage and tumor metastasis. Suppression of circDLGAP4 inhibited the biological performances of lung cancer cells. Also, there was a negative regulatory relationship between circDLGAP4 and miR-143. Inhibition of miR-143 alleviated the influences of circDLGAP4 depression on lung cancer cell biological processes. Moreover, CDK1 was discovered as a target of miR-143, and miR-143 was involved in the process of lung cancer cell biological processes through targeting CDK1. Our findings reveal that circular RNA circDLGAP4 is involved in lung cancer development through modulating microRNA-143/CDK1 axis. circDLGAP4 may serve as a potential biomarker for the diagnosis or treatment of lung cancer.