Use of magnetic capture to identify elevated levels of CCL2 following intra-articular injection of monoiodoacetate in rats. (2nd September 2020)

• Record Type: Journal Article
• Authors: Yarmola, Elena G.; Shah, Yash Y.; Lakes, Emily H.; Pacheco, Yan C.; Xie, Danny F.; Dobson, Jon; Allen, Kyle D.
• Is Part Of: Connective tissue research. Volume 61:Number 5(2020)
• Page Start: 485
• Page End: 497
• Publication Date: 2020-09-02
• Subjects: Biomarker -- monoiodoacetate -- MCP-1 or CCL2 -- synovial fluid -- magnetic particles; Connective tissues -- Periodicals; 616.770072
• DOI: 10.1080/03008207.2019.1620223
• Languages: English
• ISSNs: 0300-8207

Abstract

ABSTRACT: Purpose: Synovial fluid biomarkers help evaluate osteoarthritis (OA) development. Magnetic capture, our new magnetic nanoparticle-based technology, has proven to be effective for determining extracellular matrix fragment levels in two rat OA models. Here, the feasibility of magnetic capture for detecting monocyte chemoattractant protein-1 (MCP-1 or CCL2) is demonstrated after intra-articular injection of monoiodoacetate (MIA) in the rat knee. Methods: Forty-eight male Lewis rats received a right hind limb, intra-articular injection of MIA (1 mg in 25 µl of saline) or 25 µl of saline. Magnetic capture and lavage were performed at 7 days after injection (n = 6 per treatment per procedure), with magnetic capture additionally performed at 14 and 28 days post-injection (n = 6 per treatment per time point). CCL2 was also assessed in serum. Results: Serum CCL2 levels revealed no difference between MIA and saline animals ( p = 0.0851). In contrast, magnetic capture and lavage detected a significant increase of CCL2 in the MIA-injected knee, with the MIA-injected knee having elevated CCL2 compared to contralateral and saline-injected knees ( p = 0.00016 (contralateral) and p = 0.00016 (saline) for magnetic capture; p = 0.00023 (contralateral) and p = 0.00049 (saline) for lavage). Conclusions: Magnetic capture of CCL2 was successfully developed and applied to determine levels of CCL2 in a rat knee. Magnetic capture detected a statistically significant increase of CCL2 in MIA-injected knees compared to controls, and CCL2 levels stayed relatively stable from week 1 through week 4 post-MIA injection.