Phenotypic and genetic characterization of Pseudomonas aeruginosa isolate COP2 from the lungs of COPD patients in China. Issue 4 (26th July 2019)

• Record Type: Journal Article
• Authors: Zhao, Kelei; Yuan, Yang; Li, Jing; Pan, Wenjuan; Yan, Chaochao; Fu, Huiyao; Lin, Jiafu; Yue, Bisong; Wang, Xinrong; Gou, Xiaojun; Chu, Yiwen; Zhou, Yingshun
• Is Part Of: Pathogens and disease. Volume 77:Issue 4(2019)
• Publication Date: 2019-07-26
• Subjects: chronic obstructive pulmonary disease -- Pseudomonas aeruginosa -- phenotypic difference -- phylogenetic status -- transcriptional pattern; Medical microbiology -- Periodicals; Pathogenic microorganisms -- Periodicals; Communicable diseases -- Microbiology -- Periodicals; Communicable diseases -- Pathogenesis -- Periodicals; Host-parasite relationships -- Periodicals; Systems biology -- Periodicals; 616.904105
• DOI: 10.1093/femspd/ftz038
• Languages: English
• ISSNs: 2049-632X

Abstract

ABSTRACT: Pseudomonas aeruginosa is an important opportunistic pathogen normally associated with increasing morbidity and mortality of immunocompromised hosts with respiratory infections. The phenotypic and genetic features of P. aeruginosa from patients with chronic obstructive pulmonary disease (COPD) remain poorly understood. By using the sputum samples of 25 hospitalized COPD patients from the affiliated hospital of Southwest Medical University (China), we identified a P. aeruginosa isolate, COP2, which showed multiple antibiotic resistance and enhanced Pseudomonas quinolone signal (PQS) production but decreased motility, biofilm formation and virulence compared with the model strain PAO1. Importantly, COP2 harbored a substantial amount of mutations that might influence the functions of 1771 genes in the genome and the evolutionary status of this isolate was clearly distinct from the PAO1 lineage. Accordingly, COP2 had a discrepant transcriptional pattern relating to flagellar assembly, antibiotic resistance, biofilm and PQS production, and can increase the capacities of compound degradation in response to resource/space stresses. Therefore, the identification of COP2 in this study provides preliminary information regarding the genetic features and survival strategy of P. aeruginosa in colonizing COPD lungs and lays the foundations for further understanding of the pathogenic mechanisms of pseudomonal infections. Abstract : This study showed that enhanced antibiotic resistance, Pseudomonas quinolone signal production and compound degradation, but reduced cell motility and virulence, might contribute to the colonization of Pseudomonas aeruginosa in the lungs of patients with chronic obstructive pulmonary disease.