A dCas9-Based System Identifies a Central Role for Ctf19 in Kinetochore-Derived Suppression of Meiotic Recombination. Issue 2 (1st October 2020)

• Record Type: Journal Article
• Authors: Kuhl, Lisa-Marie; Makrantoni, Vasso; Recknagel, Sarah; Vaze, Animish N; Marston, Adele L; Vader, Gerben
• Is Part Of: Genetics. Volume 216:Issue 2(2020)
• Page Start: 395
• Page End: 408
• Publication Date: 2020-10-01
• Subjects: CRISPR -- dCas9 -- kinetochore -- meiosis -- recombination; Genetics -- Periodicals; 576.5
• DOI: 10.1534/genetics.120.303384
• Languages: English
• ISSNs: 0016-6731

Abstract

Abstract: A dCas9-based system is developed to query the regulation of kinetochore-driven meiotic recombinational control.... Abstract: In meiosis, crossover (CO) formation between homologous chromosomes is essential for faithful segregation. However, misplaced meiotic recombination can have catastrophic consequences on genome stability. Within pericentromeres, COs are associated with meiotic chromosome missegregation. In organisms ranging from yeast to humans, pericentromeric COs are repressed. We previously identified a role for the kinetochore-associated Ctf19 complex (Ctf19c) in pericentromeric CO suppression. Here, we develop a dCas9/CRISPR-based system that allows ectopic targeting of Ctf19c-subunits. Using this approach, we query sufficiency in meiotic CO suppression, and identify Ctf19 as a mediator of kinetochore-associated CO control. The effect of Ctf19 is encoded in its NH2-terminal tail, and depends on residues important for the recruitment of the Scc2 -Scc4 cohesin regulator. This work provides insight into kinetochore-derived control of meiotic recombination. We establish an experimental platform to investigate and manipulate meiotic CO control. This platform can easily be adapted in order to investigate other aspects of chromosome biology.