Use of electron microscopy to study megakaryocytes. (3rd July 2020)

• Record Type: Journal Article
• Authors: Scandola, Cyril; Erhardt, Mathieu; Rinckel, Jean-Yves; Proamer, Fabienne; Gachet, Christian; Eckly, Anita
• Is Part Of: Platelets. Volume 31:Number 5(2020)
• Page Start: 589
• Page End: 598
• Publication Date: 2020-07-03
• Subjects: Bone marrow -- correlative light electron microscopy -- electron microscopy -- megakaryocytes; Blood platelets -- Periodicals; Blood Platelets -- Periodicals; 615.39
• DOI: 10.1080/09537104.2019.1708885
• Languages: English
• ISSNs: 0953-7104

Abstract

Abstract: Electron microscopy (EM) has a long history in megakaryocyte (MK) cellular biology. This chapter shows how the electron microscope, since its first appearance almost 90 years ago, has occupied center stage in the studies of MK morphology and function. It describes some of the more productive EM techniques that have shaped our understanding of the physiology of thrombopoiesis. These include the standard transmission and scanning EM techniques as well as the new imaging methods, correlative microscopy and volume EM which provide information on the 3D organization of MKs on different scales: single organelles, whole cells and tissues. For each technique, we list the advantages and limitations, the resolution that can be achieved, the technical difficulties and the applications in MK biology.