Beneficial effect of transient desflurane inhalation on relieving inflammation and reducing signaling induced by MPTP in mice. Issue 8 (August 2022)

• Record Type: Journal Article
• Authors: Yu, Zhiyang; Qin, Guowei; Ge, Zhenzhong; Li, Weiyan
• Is Part Of: Journal of international medical research. Volume 50:Issue 8(2022)
• Publication Date: 2022-08
• Subjects: Desflurane -- MPTP -- neuroinflammation -- Parkinson's disease -- toll-like receptor -- signaling; Medicine -- Periodicals; Pharmacology -- Periodicals; 610.5
• DOI: 10.1177/03000605221115388
• Languages: English
• ISSNs: 0300-0605

Abstract

Objective: To determine if the beneficial effects of transient desflurane application mitigates inflammation and decrease associated signaling induced by 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP) in mice. Methods: Mice were induced to develop Parkinson's disease (PD) by intraperitoneal injection with MPTP for 20 consecutive days, and validated mice were randomly allocated to four groups. Collected samples from euthanized mice were designated for the following analyses: 1) immunohistochemical staining for positive dopaminergic neurons in the substantia nigra and striatum, 2) immunofluorescence staining for ionized calcium binding adaptor molecule-1 (Iba1) and glial fibrillary acid protein (GFAP), and 3) western blotting for p38, p-p38, toll-like receptor 4, and tumor necrosis factor (TNF)-α. Results: The inhalation of desflurane for 1 hour ameliorated locomotory dysfunctions of PD mice by recovering the loss of Iba1- and GFAP-positive dopaminergic neurons, deactivating microglial cells and astrocytes, and decreasing the amounts of inflammatory cytokines (TNF-α). Conclusions: These findings suggest that transient desflurane inhalation may provide some benefits for PD through ameliorating inflammation and enhancing locomotor activity.