Browning of the subcutaneous adipocytes in diet-induced obese mouse submitted to intermittent fasting. (1st August 2020)

• Record Type: Journal Article
• Authors: Marinho, Thatiany de Souza; Ornellas, Fernanda; Aguila, Marcia Barbosa; Mandarim-de-Lacerda, Carlos Alberto
• Is Part Of: Molecular and cellular endocrinology. Volume 513(2020)
• Publication Date: 2020-08-01
• Subjects: Thermogenesis -- beta3 adrenoreceptor -- Uncoupling protein 1 -- Cell proliferation -- Inflammation; β3ar beta3 adrenoreceptor -- BAT brown adipose tissue -- BM body mass -- C Control group -- CASP caspase 3 -- CIDEA cell death-inducing DFFA-like effector a -- DIO diet-induced obesity -- EE energy expenditure -- HF high-fat diet -- IF Intermittent fasting -- IL interleukin -- MCP monocyte chemoattractant protein -- PCNA proliferating cell nuclear antigen -- RQ respiratory quotient -- sWAT subcutaneous adipose tissue -- TNF tumor necrosis factor -- UCP uncoupling protein -- VEGF vascular endothelial growth factor -- WAT white adipose tissue; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2020.110872
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Purpose: We studied subcutaneous white adipose tissue (sWAT) of obese mice submitted to intermittent fasting (IF). Methods: Twelve-week-old C57BL/6 male mice received the diets Control (C) or high-fat (HF) for eight weeks (n = 20/each). Then, part of each group performed IF (24 h feeding/24 h fasting) for four weeks: C, C-IF, HF, and HF-IF (n = 10/each). Results: Food intake did not show a difference in feeding and fasting days, but HF groups had a high energy intake. IF led to multilocular adipocytes in sWAT (browning), and improved respiratory quotient on the fed day. IF decreased gene expression of Leptin, but increased Adiponectin, β3ar (beta3 adrenoreceptor), and Ucp1 (uncoupling protein). IF enhanced immunostaining of Caspase 3, Pcna (proliferating cell nuclear antigen), and UCP1 in sWAT. IF attenuated pro-inflammatory markers and pro-apoptotic markers in sWAT. Conclusions: IF in obese mice led to browning in sWAT adipocytes, enhanced thermogenesis, an improved adipose tissue pro-inflammatory profile. Graphical abstract: Obese mice have increased body mass and large unilocular adipocytes in subcutaneous white adipose tissue (sWAT). Intermittent fasting leads to adipocyte browning besides reducing the body mass and the adipocyte size, forming multilocular adipocytes in sWAT, which express brown adipocytes signs and attenuate pro-inflammatory markers. Image 1 Highlights: Browning of white adipose tissue is a target to enhance energy expenditure. The intermittent fasting acts on energy metabolism and weight loss. Obese animal adipocytes submitted to intermittent fasting showed browning. Pro-inflammatory markers are lessened in adipocytes because of intermittent fasting.