3D bioprinting modified autologous matrix-induced chondrogenesis(AMIC) technique for repair of cartilage defects. (May 2021)

• Record Type: Journal Article
• Authors: Zhou, Yang; Qin, Ran; Chen, Tong; Zhang, Kaibin; Gui, Jianchao
• Is Part Of: Materials & design. Volume 203(2021)
• Publication Date: 2021-05
• Subjects: AMIC -- 3D bioprinting -- Cartilage repair -- Chondrogenic progenitor cells -- Fibronectin -- Gradient structure; Three-dimensional 3D -- Autologous matrix induced chondrogenesis AMIC -- Chondrogenic progenitor cells CPCs -- Fibronectin FN -- Sodium alginate Alg -- Gelatin Gel -- Hyaluronic acid HA -- Proteoglycan 4 PRG4 -- COL2 Type 2 collagen -- Bone mesenchymal stem cells BMSCs -- Osteoarthritis OA -- Autologous chondrocyte implantation ACI -- Microfracture MF -- Hydroxyapatite HAP -- Extracellular matrix ECM -- Scanning electron microscopy SEM -- ANOVA Analysis of variance -- Interpenetrating Polymer Network IPN -- Enzyme linked immunosorbent assay ELISA; Materials -- Periodicals; Engineering design -- Periodicals; Matériaux -- Périodiques; Conception technique -- Périodiques; Electronic journals; 620.11
• DOI: 10.1016/j.matdes.2021.109621
• Languages: English
• ISSNs: 0264-1275

Abstract

Abstract: Three-dimensional (3D) printing technology, has achieved good results in articular cartilage damage repair, yet regeneration of hyaline cartilage similar to native cartilage and effectively performing clinical transformation remain challenging. In this study, we used 3D bioprinting technology to add chondrogenic progenitor cells (CPCs) and fibronectin (FN) to an alginate/gelatin/hyaluronic acid (Alg/Gel/HA) composite hydrogel. We used this hydrogel to prepare an active biofilm with uniform pores using modified autologous matrix-induced chondrogenesis (AMIC) technology to effectively repair cartilage defects. The Alg/Gel/HA composite hydrogel combined with FN promoted the chondrogenic differentiation of CPCs by upregulating their gene expression of collagen II, SOX9, and especially PRG4. Adjacent biofilm provided adequate mechanical support and architectural integrity, offering a stable postoperative microenvironment for bone mesenchymal stem cells (BMSCs) released from subchondral bone, and ensured that a laminar structure similar to natural hyaline cartilage was regenerated in the rat cartilage defect model. Characteristic cartilage-like lacuna structures and a gradient structure were observed in the AMIC+FN + CPCs group. We developed an effective method to regenerate full-thickness cartilage defects, this biofunctionalized cell-laden biofilm has great potential for application as a supplement to traditional AMIC technology to improve the quality of cartilage regeneration in a relatively feasible way. Graphical abstract: Unlabelled Image Highlights: Modified autologous matrix-induced chondrogenesis to repair cartilage defects. 3D printed biofilms combined chondrogenic progenitor cells. Fibronectin was added to improve the biological function of composite hydrogel. A stable postoperative microenvironment for bone mesenchymal stem cells. Reproducing layered structure similar to natural cartilage.