SDH2 is involved in proper hypha formation and virulence in Candida albicans. (August 2018)

• Record Type: Journal Article
• Authors: Bi, Shuang; Lv, Quan-Zhen; Wang, Tian-Tian; Fuchs, Beth Burgwyn; Hu, Dan-Dan; Anastassopoulou, Cleo G; Desalermos, Athanasios; Muhammed, Maged; Wu, Chin-Lee; Jiang, Yuan-Ying; Mylonakis, Eleftherios; Wang, Yan
• Is Part Of: Future microbiology. Volume 13:Number 10(2018)
• Page Start: 1141
• Page End: 1156
• Publication Date: 2018-08
• Subjects: Candida albicans -- morphogenesis -- succinate dehydrogenase -- virulence; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2018-0033
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: To investigate the role of SDH2 in Candida albicans filamentation and virulence.Materials & methods: Caenorhabditis elegans and mouse candidiasis models were used to assess the virulence of a sdh2Δ/Δ mutant. Various hypha-inducing media were used to evaluate the hyphal development of C. albicans . DCFH-DA was used to measure intracellular Reactive Oxygen Species (ROS) levels.Results: The sdh2Δ/Δ mutant was avirulent in the C. elegans model, hypovirulent in a murine candidiasis model, and defective to form filaments both in vitro and in vivo . Intracellular ROS level increased in the sdh2Δ/Δ mutant, and the filamentation defects of sdh2Δ/Δ were rescued by decreasing intracellular ROS.Conclusion: SDH2 plays an important role in C. albicans filamentation and virulence probably through affecting intracellular ROS.