Biosynthesis, characterization and leishmanicidal activity of a biocomposite containing AgNPs-PVP-glucantime. (February 2018)

• Record Type: Journal Article
• Authors: Gélvez, Ana Patricia Cacua; Farias, Luis Henrique Seabra; Pereira, Victor Soares; da Silva, Inaldo Claudio Martins; Costa, Ana Carolina; Dias, Carmen Gilda Barroso Tavares; Costa, Roseane Maria Ribeiro; da Silva, Silvia Helena Marques; Rodrigues, Ana Paula Drummond
• Is Part Of: Nanomedicine. Volume 13:Number 4(2018)
• Page Start: 373
• Page End: 390
• Publication Date: 2018-02
• Subjects: biosynthesis -- silver nanoparticles -- Leishmania; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2017-0285
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Development of functionalized nanocomposites containing AgNPs-PVP-Glucantime ® to evaluate their leishmanicidal activity as a novel method for improving the pharmacological properties of the drug Glucantime ® against extracellular promastigotes and intracellular amastigotes of Leishmania amazonensis in vitro to treat cutaneous leishmaniasis.Materials & methods: The silver nanoparticles and nanocomposites prepared containing silver nanoparticles, polyvinylpyrrolidone and different amounts of Glucantime were characterized using transmission electron microscopy, x-ray diffraction, energy-dispersive x-ray spectroscopy and ζ potential analysis; in addition, the in vitro cytotoxicity was evaluated.Results: The nanocomposites showed an inhibitory effect on the cellular viability of promastigote forms, with values of 47.06, 51.71 and 65.67% for nanocomposite1, nanocomposite2 and nanocomposite3, respectively, as well as a dose-dependent decrease in the infectivity index, with values of 33.33 and 23% for nanocomposite2 and nanocomposite3, respectively.Conclusion: The proposed nanocomposite reveals leishmanial activity and the absence of cytotoxicity in macrophages. Further investigations will be conducted in vivo .