Mycosynthesis of bactericidal silver and polymorphic gold nanoparticles: physicochemical variation effects and mechanism. (January 2018)

• Record Type: Journal Article
• Authors: Singh, Dheeraj Kumar; Kumar, Jitendra; Sharma, Vijay Kumar; Verma, Satish Kumar; Singh, Arti; Kumari, Puja; Kharwar, Ravindra Nath
• Is Part Of: Nanomedicine. Volume 13:Number 2(2018)
• Page Start: 191
• Page End: 207
• Publication Date: 2018-01
• Subjects: biosynthesis mechanism -- metal nanoparticles -- mycofabrication; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2017-0235
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Extracellular synthesis of silver and gold nanoparticles using aqueous cell-free filtrate (CFF) of endophytic Chaetomium globosum and characterization of its bioactive proteins.Methods: Temperature and pH gradients were used to assess their effects on dimensions of NPs. NPs were tested in vivo for antibacterial activity. MALDI-TOF-MS/MS was used for characterization of CFF proteins.Results: Fungal CFF fabricated nanoparticles of various shape under varied physicochemical conditions. Silver nanoparticles showed significantly (p ≤ 0.5) enhanced antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae compared with AgNO3 . Two prominent CFF proteins showed homology with benzoate 4-monooxygenase cytochrome P450 and ubiquinol-cytochrome c reductase.Conclusion: The study achieved controlled mycosynthesis of NPs and explains the hitherto poorly known mechanism of reduction, stabilization and antibacterial activity of nanoparticles.