Ζ potential changing nanoparticles as cystic fibrosis transmembrane conductance regulator gene delivery system: an in vitro evaluation. (November 2017)

• Record Type: Journal Article
• Authors: Prüfert, Felix; Bonengel, Sonja; Köllner, Saskia; Griesser, Janine; Wilcox, Matthew D; Chater, Peter I; Pearson, Jeffrey P; Bernkop-Schnürch, Andreas
• Is Part Of: Nanomedicine. Volume 12:Number 22(2017)
• Page Start: 2713
• Page End: 2724
• Publication Date: 2017-11
• Subjects: cystic fibrosis transmembrane conductance regulator -- gene delivery -- phosphatase -- phosphotyrosine -- ζ potential changing nanoparticles; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2017-0115
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: Aim of the study was the development of ζ potential changing nanoparticles as gene delivery system for the cystic fibrosis transmembrane conductance regulator gene.Methods: Chitosan and carboxymethyl cellulose were modified with phosphotyrosine, a substrate for the brush border enzyme alkaline phosphatase. With these synthesized derivatives, different nanoparticle formulations, including the cystic fibrosis transmembrane conductance regulator gene were prepared by ionic gelation.Results: A change from negative to positive ζ potential after enzymatic cleavage could be observed. Transfection studies with HEK-293 and Caco-2 cells showed transfection rates comparable to Lipofectamine 2000. Transfection efficiencies were significantly decreased when phosphate cleavage and thus ζ potential change was inhibited by phosphatase inhibitor.Conclusion: The developed nanoparticles represent a promising gene delivery system.