Nanofiber-based sutures induce endogenous antimicrobial peptide. (November 2017)

• Record Type: Journal Article
• Authors: Chen, Shixuan; Ge, Liangpeng; Gombart, Adrian F; Shuler, Franklin D; Carlson, Mark A; Reilly, Debra A; Xie, Jingwei
• Is Part Of: Nanomedicine. Volume 12:Number 21(2017)
• Page Start: 2597
• Page End: 2609
• Publication Date: 2017-11
• Subjects: 25-hydroxyvitamin D3 -- co-delivery -- electrospun nanofibers -- pam3Cys-Ser-(Lys)4 peptide -- surgical site infection -- sutures; Nanotechnology -- Periodicals; Medical technology -- Periodicals; Nanotechnology -- Therapeutic use -- Periodicals; 610.28
• DOI: 10.2217/nnm-2017-0161
• Languages: English
• ISSNs: 1743-5889

Abstract

Aim: The aim of this study was to develop nanofiber-based sutures capable of inducing endogenous antimicrobial peptide production.Methods: We used co-axial electrospinning deposition and rolling to fabricate sutures containing pam3CSK4 peptide and 25-hydroxyvitamin D3 (25D3 ).Results: The diameters and mechanical properties of the sutures were adjustable to meet the criteria of United States Pharmacopeia designation. 25D3 exhibited a sustained release from nanofiber sutures over 4 weeks. Pam3CSK4 peptide also showed an initial burst followed by a sustained release over 4 weeks. The co-delivery of 25D3 and pam3CSK4 peptide enhanced cathelicidin antimicrobial peptide production from U937 cells and keratinocytes compared with 25D3 delivery alone. In addition, the 25D3 /pam3CSK4 peptide co-loaded nanofiber sutures did not significantly influence proliferation of keratinocytes, fibroblasts, or the monocytic cell lines U937 and HL-60.Conclusion: The use of 25D3 /pam3CSK4 peptide co-loaded nanofiber sutures could potentially induce endogenous antimicrobial peptide production and reduce surgical site infections.