Adipose-derived stem-cell-implanted poly(ϵ-caprolactone)/chitosan scaffold improves bladder regeneration in a rat model. (2nd May 2018)

• Record Type: Journal Article
• Authors: Zhou, Zhe; Yan, Hao; Liu, Yidong; Xiao, Dongdong; Li, Wei; Wang, Qiong; Zhao, Yang; Sun, Kang; Zhang, Ming; Lu, Mujun
• Is Part Of: Regenerative medicine. Volume 13:Number 3(2018)
• Page Start: 331
• Page End: 342
• Publication Date: 2018-05-02
• Subjects: ϵ-caprolactone -- adipose-derived stem cells -- bladder augmentation -- chitosan -- rat model -- regeneration -- scaffolds -- stem cells -- tissue engineering -- urinary bladder; Cellular therapy -- Periodicals; Stem cells -- Transplantation -- Periodicals; Degeneration (Pathology) -- Treatment -- Periodicals; Regeneration (Biology) -- Periodicals; 611.018
• DOI: 10.2217/rme-2017-0120
• Languages: English
• ISSNs: 1746-0751

Abstract

Aim: The study investigated the feasibility of seeding adipose-derived stem cells (ASCs) onto a poly(ϵ-caprolactone)/chitosan (PCL/CS) scaffold for bladder reconstruction using a rat model of bladder augmentation.Materials & methods: In the experimental group, the autologous ASCs were seeded onto the PCL/CS scaffold for bladder augmentation. An unseeded scaffold was used for bladder augmentation as control group. The sham group was also set.Result: 8 weeks after implantation, more densely smooth muscles were detected in the experimental group with a larger bladder capacity and more intensive blood vessels. Immunofluorescence staining demonstrated that some of the smooth muscle cells were transdifferentiated from the ASCs.Conclusion: Our findings indicated that ASC-seeded PCL/CS may be a potential scaffold for bladder tissue engineering.