Preparation monoclonal β-type anti-idiotype antibody of zearalenone and development of green ELISA quantitative detecting technique. Issue 4 (20th April 2020)

• Record Type: Journal Article
• Authors: Shi, Luhuai; Yu, Tao; Luo, Miner; Wang, Hong
• Is Part Of: Preparative biochemistry & biotechnology. Volume 50:Issue 4(2020)
• Page Start: 419
• Page End: 424
• Publication Date: 2020-04-20
• Subjects: Zearalenone -- monoclonal antibody -- β-type anti-idiotype antibody -- indirect competitive enzyme-linked immunosorbent assay; Biochemistry -- Technique -- Periodicals; Biotechnology -- Technique -- Periodicals; Biochemistry -- methods -- Periodicals; Biotechnology -- methods -- Periodicals; 660.6
• DOI: 10.1080/10826068.2019.1703195
• Languages: English
• ISSNs: 1082-6068

Abstract

Abstract: Immunoassay has been widely used in the screening of mycotoxins, which may be hazardous to the operator or the environment. This study was to develop a green way to measure zearalenone (ZEN) with a monoclonal β-type anti-idiotype antibody (Ab2β) against ZEN in place of ZEN standard. Six monoclonal β-type anti-idiotype antibodies were prepared. The 50% inhibitory concentration (IC50 ) value to ZEN of the six antibodies was between 34.45 ± 1.12–182.12 ± 15.40 nM. A green ELISA was then developed and validated. The quantitative conversion formula between ZEN and the monoclonal Ab2β against ZEN was y = 0.092 x 0.722, R 2 = 0.990. The working range was 2.63–100.64 ng ml −1 . The recovery rate in spiked feed samples was from 82.15% to 102.79%, and the within-assay and between-assay coefficient variation (CV) level were less than 10.00%. A good correlation was obtained by high-performance liquid chromatography method (HPLC) to validate the developed method.