Molecular characterization of hepatitis D virus genotypes circulating in Iran. (23rd February 2018)

• Record Type: Journal Article
• Authors: Meysami, Parisa; Alavian, Seyed Moayed; Monavari, Seyed Hamidreza; Mozhgani, Sayed-Hamidreza; Taghizadeh, Morteza; Farahani, Maryam; Ansari, Nastaran; Keyvani, Hossein
• Is Part Of: Future virology. Volume 13:Number 4(2018)
• Page Start: 265
• Page End: 273
• Publication Date: 2018-02-23
• Subjects: genotype -- hepatitis D virus -- HDV -- Iran -- phylogenetic analysis -- RFLP; Virology -- Periodicals; Virus diseases -- Treatment -- Periodicals; 616.9101
• DOI: 10.2217/fvl-2017-0156
• Languages: English
• ISSNs: 1746-0794

Abstract

Aim: To determine the molecular epidemiology and characterization of hepatitis D virus (HDV) genotypes circulating in different provinces of Iran.Patients & methods: In this study, the presence of HDV RNA was tested in sera that were positive for hepatitis B surface antigen and HDV antibody by nested-PCR. HDV genotypes were subsequently analyzed using restriction fragment length polymorphism (RFLP) assay and then confirmed by sequencing.Results: 86.5% of positive PCR patients had genotype I and 8.1% had genotype II while the genotype of 5.4% of the patients remained undetermined by RFLP. Sequencing followed by phylogenetic analysis demonstrated that all the Iranian isolates were from genotype I.Conclusion: Although analyzing the RFLP of RT-PCR is a simpler method, the gold standard of genotyping of HDV is the phylogenetic analysis based on sequencing.