Molecular determinants possibly involved in the adaptation of pandemic A(H1N1)09 influenza virus to a new host. (September 2017)

• Record Type: Journal Article
• Authors: Prokopyeva, Elena; Romanovskaya, Alesia; Sharshov, Kirill; Sobolev, Ivan; Alekseev, Alexander; Durymanov, Alexander; Shestopalov, Alexander
• Is Part Of: Future virology. Volume 12:Number 9(2017)
• Page Start: 491
• Page End: 498
• Publication Date: 2017-09
• Subjects: A(H1N1)pdm09 virus adaptation -- HA -- IHC mice model -- NA -- PA -- PB1 -- PB2; Virology -- Periodicals; Virus diseases -- Treatment -- Periodicals; 616.9101
• DOI: 10.2217/fvl-2017-0026
• Languages: English
• ISSNs: 1746-0794

Abstract

Aim: To determine the molecular basis of A(H1N1)pdm09 virus adaptation to a new host.Methods: The pandemic virus A/Russia/01/2009 was adapted to mice by serial lung-to-lung passages. The growth properties, pathogenicity and genome sequences of the mouse-adapted virus were compared with those of the parental strain.Results: The A(H1N1)pdm09 virus caused mouse death after only six lung-to-lung passages. Mutations in the influenza virus antigen were found in the brain, liver, kidney and in intestine by immunohistochemistry.Conclusion: Our findings suggest that multiple mutations in the viral genome promote rapid adaptation of the A(H1N1)pdm09 virus and cause generalized infection in mice.