MiRNA-20a upregulates TAK1 and increases proliferation in osteosarcoma cells. (February 2018)

• Record Type: Journal Article
• Authors: Yuan, Guangke; Zhao, Yanqing; Wu, Dongjin; Gao, Chunzheng; Jiao, Zhaode
• Is Part Of: Future oncology. Volume 14:Number 5(2018)
• Page Start: 461
• Page End: 469
• Publication Date: 2018-02
• Subjects: apoptosis -- cell proliferation -- cisplatin -- doxorubicin -- drug resistance -- miR-20a -- NF-kB pathway -- osteosarcoma -- synergism -- TAK1; Oncology -- Periodicals; 616.99405
• DOI: 10.2217/fon-2017-0490
• Languages: English
• ISSNs: 1479-6694

Abstract

Aim: The aim of this study is to explore the function of miR-20a in osteosarcoma.Materials & methods: miR-20a expression was measured by real-time PCR. miR-20a mimics, inhibitor and scramble siRNA were transfected into osteosarcoma cells to observe effects on colony formation and tumor growth. Moreover, relationships of miR-20a with TAK1 were investigated by western blot and luciferase activity.Results: We found that miR-20a was downregulated in osteosarcoma, and overexpression of miR-20a reduced colony formation and tumor growth. Furthermore, the data revealed that the function of miR-20a was probably exerted via targeting the TAK1 expression. Overexpression of miR-20a sensitizes the osteosarcoma cells to chemotherapeutic drugs.Conclusion: Our data identify the role of miR-20a in osteosarcoma growth, indicating its potential application in chemotherapy.