Extracellular granzyme A in amniotic fluid is elevated in the presence of sterile intra-amniotic inflammation in preterm prelabor rupture of membranes. (2nd September 2022)

• Record Type: Journal Article
• Authors: Spacek, Richard; Musilova, Ivana; Andrys, Ctirad; Soucek, Ondrej; Burckova, Hana; Pavlicek, Jan; Pliskova, Lenka; Bolehovska, Radka; Kacerovsky, Marian
• Is Part Of: Journal of maternal-fetal & neonatal medicine. Volume 35:Number 17(2022)
• Page Start: 3244
• Page End: 3253
• Publication Date: 2022-09-02
• Subjects: Adaptive immunity -- intra-amniotic inflammation -- microbial invasion of the amniotic cavity -- preterm delivery -- protease -- T cells -- NK cells; Obstetrics -- Periodicals; Perinatology -- Periodicals; Infants (Newborn) -- Diseases -- Periodicals; Neonatology -- Periodicals; 618.2
• DOI: 10.1080/14767058.2020.1817895
• Languages: English
• ISSNs: 1476-7058

Abstract

Abstract: Introduction: To determine the levels of granzyme A in amniotic fluid in pregnancies complicated by preterm prelabor rupture of membranes (PPROM), based on the presence of microbial invasion of the amniotic cavity (MIAC) and/or intra-amniotic inflammation (IAI). Methods of study: A total of 166 women with singleton pregnancies complicated by PPROM were included. Amniocentesis was performed at the time of admission and assessments of MIAC (using both cultivation and non-cultivation techniques) and IAI (interleukin-6 in amniotic fluid) were performed on all subjects. Based on the presence/absence of MIAC and IAI, the women were further divided into the following subgroups: intra-amniotic infection, sterile IAI, colonization, and absence of both MIAC and IAI. Amniotic fluid granzyme A levels were assessed using ELISA. Results: Women with MIAC had lower levels of granzyme A in the amniotic fluid than women without this condition (with MIAC: median 15.9 pg/mL vs. without MIAC: median 19.9 pg/mL, p = .03). Women with sterile IAI had higher amniotic fluid granzyme A levels than women with intra-amniotic infection, colonization and women with the absence of either MIAC or IAI (intra-amniotic infection: median 15.6 pg/mL; sterile IAI: median 31.8 pg/mL; colonization: median 16.9 pg/mL; absence of both MIAC and IAI: median 18.8 pg/mL; p = .02). Conclusions: The presence of sterile IAI was associated with elevated levels of granzyme A in amniotic fluid.