Potential of novel food-borne Lactobacillus isolates against the honeybee pathogen Paenibacillus larvae. Issue 9 (1st September 2020)

• Record Type: Journal Article
• Authors: Lazzeri, Anna Marta; Mangia, Nicoletta P.; Mura, Maria Elena; Floris, Ignazio; Satta, Alberto; Ruiu, Luca
• Is Part Of: Biocontrol science and technology. Volume 30:Issue 9(2020)
• Page Start: 897
• Page End: 908
• Publication Date: 2020-09-01
• Subjects: Insect -- Antibiotic -- Antimicrobial -- Plantaricin -- Lactobacillus plantarum; Pests -- Biological control -- Periodicals; Weeds -- Biological control -- Periodicals; Pesticides -- Environmental aspects -- Periodicals; Plants, Protection of -- Periodicals; Plants -- Biological control -- Periodicals; Agricultural pests -- Control -- Periodicals; Pest Control, Biological -- Periodicals; 632.96
• DOI: 10.1080/09583157.2020.1769556
• Languages: English
• ISSNs: 0958-3157

Abstract

ABSTRACT: An in vitro study was conducted to screen for the inhibitory potential of lactic acid bacteria (LAB), isolated from fermented food matrices, against the honeybee American Foulbrood agent Paenibacillus larvae . Various strains of Lactobacillus plantarum showed significant antagonism associated with their cell-free culture supernatant (CFS). The characterisation of this inhibition was based on assays and analyses involving different fraction and extracts. A significant effect was associated with media acidification resulting from LAB growth. However, this antimicrobial activity was found to be heat-stable and specific experiments suggested the implication of small antimicrobial peptides and/or antibiotics. Accordingly, a possible role of plantaricins released by L. plantarum in the culture supernatant was confirmed by the expression of different plantaricin-related genes. The results obtained in this study support further investigations on LAB strains as biological control agents for application in the apiary to preserve and improve honeybee health.