Furin‐Mediated Self‐Assembly of Olsalazine Nanoparticles for Targeted Raman Imaging of Tumors. (26th January 2021)

• Record Type: Journal Article
• Authors: Yuan, Yue; Raj, Piyush; Zhang, Jia; Siddhanta, Soumik; Barman, Ishan; Bulte, Jeff W. M.
• Is Part Of: Angewandte Chemie. Volume 133:Number 8(2021)
• Page Start: 3969
• Page End: 3973
• Publication Date: 2021-01-26
• Subjects: furin -- nanoparticles -- olsalazine -- Raman spectroscopy -- self-assembly; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.202014839
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Olsalazine (Olsa) is a broad‐spectrum anti‐cancer agent acting as a DNA‐methylation inhibitor. When conjugated to 2‐cyano‐6‐aminobenzothiazole and a peptide substrate specific for the tumor‐overexpressed enzyme furin, it can self‐assemble into nanoparticles that can be detected by chemical‐exchange saturation‐transfer magnetic‐resonance imaging (CEST MRI). We report here that these nano‐assemblies can also be detected with high specificity in furin‐overexpressing tumor cells by Raman spectroscopy with a distinct scattering signature and demonstrate the utility of this sensing mechanism in vitro and in vivo. Our findings suggest that Raman spectroscopy could be used for high‐resolution image‐guided surgery to precisely delineate tumor margins during and after resection in real‐time as well as to determine microscopic tumor invasion and multifocal locoregional tumor spread, which are currently impossible to visualize with available imaging technologies, including CEST MRI. Abstract : Based on a furin‐mediated CBT‐Cys click condensation reaction followed by intracellular nanoparticle self‐assembly, we report the development of a new furin‐specific Raman reporter Olsa‐RVRR and demonstrate its in‐vitro and in‐vivo utility in tumor detection.