High-affinity recombinant full-length antibody-based immunochromatographic strip assay for rapid and reliable detection of pyraclostrobin residues in food samples. Issue 1 (1st January 2020)

• Record Type: Journal Article
• Authors: Liu, Ying; Jiao, Shasha; Chang, Yunyun; Lu, Xinying; Liu, Pengyan; Zhao, Ying; Zha, Changchun; Shen, Lirong; Guo, Yirong; Zhu, Guonian
• Is Part Of: Food and agricultural immunology. Volume 31:Issue 1(2020)
• Page Start: 985
• Page End: 1003
• Publication Date: 2020-01-01
• Subjects: Full-length recombinant antibody -- gold nanoparticles -- immunochromatographic strip -- pyraclostrobin -- sample detection; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2020.1797640
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: In this study, a rapid and reliable gold nanoparticles immunochromatographic strip (GNPs-ICS) assay based on a full-length recombinant antibody (rAb) was established for pyraclostrobin detection in food samples. First, a hybridoma PY-C7 secreting pyraclostrobin-specific monoclonal antibody (mAb) was produced. The variable region sequences of PY-C7-mAb were obtained by PCR and Sanger sequencing and used for the rAb expression in HEK293(F) cells. By GNPs-ICS assay, the visual LOD of pyraclostrobin standards was 0.1 mg L −1 (rAb) and 0.2 mg L −1 (mAb). Validated by recovery test, the visual LOQ of pyraclostrobin could meet the MRLs of Chinese food safety standard in strawberry, banana and Chinese cabbage samples by GNPs-mAb/rAb-ICS, in which the rAb showed better sensitivity and stability. Moreover, the GNPs-rAb-ICS showed comparable reliability with LC-MS/MS for actual sample detection. Above all, GNPs-ICS assay based on the full-length rAb was qualified for rapid detection of pyraclostrobin in food samples. GRAPHICAL ABSTRACT: