A novel reversible fluorescent probe for Zinc(II) ion and bioimaging in living cells. Issue 7 (2nd July 2020)

• Record Type: Journal Article
• Authors: Chang, Yongxin; Li, Bai; Mei, Huihui; Xu, Kuxi; Xie, Xinmei; Yang, Li
• Is Part Of: Supramolecular chemistry. Volume 32:Issue 7(2020)
• Page Start: 393
• Page End: 402
• Publication Date: 2020-07-02
• Subjects: Zn2+ -- fluorescent probe -- cells imaging; Macromolecules -- Periodicals; Supramolecular chemistry -- Periodicals; 547.005
• DOI: 10.1080/10610278.2020.1749627
• Languages: English
• ISSNs: 1061-0278

Abstract

ABSTRACT: A simple Schiff-base fluorescent probe (L) was synthesised for selective detection of Zn 2+ ion in CH3 CN/HEPES solution (1/1, 10.0 μM, pH = 7.0). The probe L showed highly fluorescence enhancement towards Zn 2+ over other metal ions with obvious fluorescence colour change from faint yellow to blue, which should be attributed to the formation of a 1:2 ligand-metal complex resulting in the inhibition of intramolecular charge transfer (ICT) and the chelation-enhanced fluorescence (CHEF) process. The bonding mechanism was confirmed by UV-vis, fluorescence, HRMS, 1 H NMR experiments and theoretical calculation. Reversibility of the probe in its binding with Zn 2+ ion was established in the presence of EDTA. Furthermore, the fluorescence imaging indicated that probe L could be used to effectively identify Zn 2+ in living cells. Graphical abstract: A novel simple Schiff-base fluorescent probe (L) has been prepared for selective detection of Zn 2+ ion in CH3 CN/HEPES solution (1/1, 10.0 μM HEPES, pH 7.0). The probe L showed highly fluorescence enhancement to Zn 2+ over other metal ions with obvious fluorescence colour change from colourless to yellow, which might be attributed to the formation of a 1:2 ligand-metal complex resulting in the inhibition of intramolecular charge transfer (ICT) and the chelation-enhanced fluorescence (CHEF) process. The bonding mechanism was confirmed by UV-vis, fluorescence, 1 H NMR experiment and theoretical calculation. Reversibility of the probe in its binding with Zn 2+ ion is established in the presence of EDTA. Furthermore, the fluorescence imaging indicated that probe L could be used to effectively identify Zn 2+ in living cells.