Accurate differentiation of novel Staphylococcus argenteus from Staphylococcus aureus using MALDI-TOF MS. (July 2018)

• Record Type: Journal Article
• Authors: Chen, Shey-Ying; Lee, Hao; Teng, Shih-Hua; Wang, Xiao-Mei; Lee, Tai-Fen; Huang, Yu-Chung; Liao, Chun-Hsing; Teng, Lee-Jene; Hsueh, Po-Ren
• Is Part Of: Future microbiology. Volume 13:Number 9(2018)
• Page Start: 997
• Page End: 1006
• Publication Date: 2018-07
• Subjects: Bruker Biotyper MALDI-TOF MS -- classification model -- matrix-assisted laser desorption/ionization-time of flight mass spectrometry -- Staphylococcus argenteus -- Staphylococcus aureus; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2018-0015
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: We evaluated a Staphylococcus argenteus- specific diagnostic profile of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for accurate identification of this novel bacterium.Materials & methods: Staphylococcus argenteus was identified based on negative crtM gene and presence of specific sequence types. A classification model was generated by cluster analysis of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer results and ClinProTools software for 25 S. argenteus and 25 methicillin-susceptible S. aureus (MSSA). The performance of the classification model was validated against 72 S. argenteus and 72 MSSA isolates.Results: With cluster analysis and classification model, differentiation of 72 S. argenteus from 72 MSSA had 100.0% accuracy by chemical extraction method and 87.5% sensitivity and 100.0% specificity by direct smear method.Conclusion: The classification model could accurately differentiate S. argenteus from MSSA.