Bactericidal synergism between antibiotics and phage endolysin Cpl-711 to kill multidrug-resistant pneumococcus. (September 2018)

• Record Type: Journal Article
• Authors: Letrado, Patricia; Corsini, Bruno; Díez-Martínez, Roberto; Bustamante, Noemí; Yuste, José E.; García, Pedro
• Is Part Of: Future microbiology. Volume 13:Number 11(2018)
• Page Start: 1215
• Page End: 1223
• Publication Date: 2018-09
• Subjects: antibiotic multidrug-resistant -- endolysin -- mouse model -- Streptococcus pneumoniae -- synergy -- zebrafish model; Microbiology -- Research -- Periodicals; 616.9041
• DOI: 10.2217/fmb-2018-0077
• Languages: English
• ISSNs: 1746-0913

Abstract

Aim: To test the synergistic effect of Cpl-711 endolysin and antibiotics for antipneumococcal activity.Materials & methods: A combination of Cpl-711 and different antibiotics (amoxicillin, cefotaxime, levofloxacin and vancomycin) was tested in a checkerboard assay against several multidrug-resistant Streptococcus pneumoniae strains. Mouse and zebrafish models of pneumococcal sepsis were used to confirm the in vitro data.Results: The activity of Cpl-711 combined with amoxicillin or cefotaxime was synergistic in the bactericidal effect against a serotype 23F multiresistant clinical isolate of S. pneumoniae . Synergy between Cpl-711 and cefotaxime was validated using both mouse and zebrafish models.Conclusion: Combination of Cpl-711 and cefotaxime may help in the treatment of diseases caused by multiresistant pneumococcal strains. Graphical abstract: